human prostate cell pnt1a  (Procell Inc)

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: The secretion of testosterone ( a , b ), estradiol ( c , d ), and progesterone ( e , f ) after 48 h of exposure to AOH and DHEA, evaluated by ELISA tests in PC3 and PNT1A cells. Results are presented as mean ± SE. One-way ANOVA was used to perform statistical analysis of the results, and p < 0.05 was considered statistically significant. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of CAV-1. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, CAV-1—caveolin 1.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce apoptosis after 48 h of exposure in PC3 and PNT1A cells ( a ). Analysis of apoptotic cells based on flow cytometry with representative results ( b ). One-way ANOVA was used for statistical analysis. Results are presented and mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001, as compared to the control. Representative results of Western blot analysis of CASP3 ( c ). Analysis of the relative expression of ANX5a ( d ) and TP53 genes in normal and prostate cancer cell lines ( e ). AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Flow Cytometry, Control, Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of Casp3 and Cleaved Casp3. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce changes in cell cycle progression after 48 h of exposure in PNT1A ( a ) and PC3 cells ( b ). Analysis based on the results obtained in flow cytometry. Analysis of the relative expression of CDKN1A ( c ) and CDK2 genes in normal and prostate cancer cell lines ( d ). One-way ANOVA was used for statistical analysis. The results are presented as mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control. The results were acquired in three independent experiments.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Flow Cytometry, Expressing, Control

Journal: Toxins

Article Title: Estrogen Receptor β Participates in Alternariol-Induced Oxidative Stress in Normal Prostate Epithelial Cells

doi: 10.3390/toxins13110766

Figure Lengend Snippet: Blocking of ERβ decreases AOH- induced oxidative stress in PNT1A cells. ( A ) ROS positive cells counted with Muse ® Cell Analyzer. ( B ) Representative results of Western blot analysis of SOD1 expression. All results are expressed as mean ± SE. One-way ANOVA was used for statistical analysis. p < 0.05 was considered as statistically significant, * p < 0.05, ** p < 0.01, *** p < 0.001, ## p < 0.01, ### p < 0.001 as compared to Cnt PHTPP. AOH—alternariol, PHTPP—ERβ inhibitor, Cnt—control. The experiments were run in 3 replications.

Article Snippet: PNT1A, normal human prostatic cell line, was supplied by the European Collection of Authenticated Cell Cultures (ECACC, Sigma-Aldrich, Saint Louis, MO, USA).

Techniques: Blocking Assay, Western Blot, Expressing, Control

Journal: Toxins

Article Title: Estrogen Receptor β Participates in Alternariol-Induced Oxidative Stress in Normal Prostate Epithelial Cells

doi: 10.3390/toxins13110766

Figure Lengend Snippet: AOH induces DNA damage in PNT1A cells. ( A ) DNA damage was counted on Muse ® Cell Analyzer and expressed as % of cells gated. ( B ) Relative expression of PARP1 obtained in RT-qPCR. The results are expressed as mean ± SE. ( C ) DAPI staining of the nuclei of cells. White arrows point at the cells with changed of fragmented nuclei. ( D ) Representative results of Western blot analysis of the expression of cleaved-PARP1 protein. One way ANOVA was used for statistical analysis. p < 0.05 was considered as statistically significant. * p < 0.05, *** p < 0.001 as compared to Cnt, ### p < 0.001 as compared to Cnt PHTPP. AOH—alternariol, PHTPP—ERβ inhibitor, Cnt—control. The experiments were run in 3 replications.

Article Snippet: PNT1A, normal human prostatic cell line, was supplied by the European Collection of Authenticated Cell Cultures (ECACC, Sigma-Aldrich, Saint Louis, MO, USA).

Techniques: Expressing, Quantitative RT-PCR, Staining, Western Blot, Control

Journal: Toxins

Article Title: Estrogen Receptor β Participates in Alternariol-Induced Oxidative Stress in Normal Prostate Epithelial Cells

doi: 10.3390/toxins13110766

Figure Lengend Snippet: AOH decreases migration of PNT1A cells. ( A ) Migration of cells was evaluated with scratch assay. ( B ) Zymography was used to evaluate the activity of MMP-9 and MMP-2. The results are expressed as mean ± SE. One- way ANOVA was used for statistical analysis. p < 0.05 was considered as statistically significant. ** p < 0.01 as compared to Cnt, ### p < 0.001 as compared to Cnt PHTPP. AOH—alternariol, PHTPP—ERβ inhibitor, Cnt—control. The experiments were run in 3 replications.

Article Snippet: PNT1A, normal human prostatic cell line, was supplied by the European Collection of Authenticated Cell Cultures (ECACC, Sigma-Aldrich, Saint Louis, MO, USA).

Techniques: Migration, Wound Healing Assay, Zymography, Activity Assay, Control